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ErbB3/Her3 His Tag Protein, Human

ErbB3/Her3 His Tag Protein, Human

Catalog Number: UA016070 Brand: UA BIOSCIENCE
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$225 USD
$225 USD
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Product Details

Product Specification


Species Human
Synonyms ErbB3, Her3, FERLK, LCCS2, VSCN1
Accession P21860
Amino Acid Sequence

Ser20-Thr643 with His Tag at C-terminal
Expression System HEK293
Molecular Weight 100-130kDa (Reducing)
Purity ＞95% by SDS-PAGE & HPLC
Conjugation Unconjugated
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Ma, J., et al. (2026). HER3 beyond the canonical paradigm: a versatile signaling hub in oncogenesis and therapeutic resistance. Journal of Translational Medicine, 24(1), 123.
2. Wang, Y., & Zhang, X. (2024). HER3: Updates and current biology function, targeted therapy and pathologic detecting methods. Life Sciences, 357, 123087.
3. Kiavue, N., et al. (2020). ERBB3 mutations in cancer: biological aspects, prevalence and therapeutics. Oncogene, 39(3), 487-502.

Background

Human Epidermal Growth Factor Receptor 3 (HER3/ErbB3) is a distinctive member of the epidermal growth factor receptor (EGFR/ErbB) family, which plays a central role in cell proliferation, differentiation, migration, and survival. Its most prominent feature is that its intracellular kinase domain exhibits extremely low, if not absent, catalytic activity compared to other family members (EGFR, HER2, HER4). Consequently, HER3 cannot signal alone and must heterodimerize with other active family members (primarily HER2) to be activated and initiate downstream signaling pathways.

Structurally, HER3, like other ErbB family members, consists of an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular domain containing the kinase domain. Its extracellular domain specifically binds to ligands such as Neuregulin (NRG), triggering a conformational change that exposes its dimerization arm, enabling it to bind to EGFR or HER2.

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SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of ErbB3/Her3 His Tag Protein, Human is more than 95% determined by SEC-HPLC.

ELISA

Immobilized ErbB3 / Her3 His Tag Protein, Human (Cat. No.UA016070) at 2.0μg/mL (100μL/well) can bind Anti-Human HER3 Monoclonal Antibody (Patritumab) with EC50 of 0.08-0.16ng/mL.

Immobilized ErbB3 / Her3 His Tag Protein, Human (Cat. No. UA016070) at 2.0μg/mL (100μL/well) can bind Human NRG1 Beta 1 Protein, Fc Tag, premium grade with EC50 of 1.90-2.40 ng/mL.