IHC shows positive staining in paraffin-embedded human breast. Anti- ErbB3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ErbB3 Recombinant Rabbit mAb (SDT-991-73-2)
ErbB3 Recombinant Rabbit mAb (SDT-991-73-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ErbB3 |
| Synonyms | Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, HER3 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P21860 |
| Clone Number | SDT-991-73-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:200-1:500 | null |
Background
HER3/ERBB3 is a member of the family of ERBB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. The phosphoric acidization of ERBB3 depends on the combination of other ERBB tyrosine kinase. When combined with ligands, an heterodimers is formed between ERBB3 and other ERBB proteins. ERBB3 is widely expressed in human adult tissues, including cells of the gastrointestinal, urinary, respiratory, reproductive tracts, skin, endocrine and nervous systems. ERBB3 is highly expressed in many cancer cells, such as breast, ovarian, lung, colorectal, melanoma, head and neck, cervical and prostate cancers.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- ErbB3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- ErbB3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
