IF shows positive staining in paraffin-embedded human breast cancer. Anti- ErbB2 (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
ErbB2 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (S-069-57)
ErbB2 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (S-069-57)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ErbB2 |
| Synonyms | HER2, MLN19, NEU, NGL, CD340,ERBB2 |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | P04626 |
| Clone Number | S-069-57 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:500 |
Background
Receptor tyrosine-protein kinase erbB-2 is a protein that in humans is encoded by the ERBB2 gene. ERBB is abbreviated from erythroblastic oncogene B. The human protein is also frequently referred to as HER2 (human epidermal growth factor receptor 2) or CD340 (cluster of differentiation 340). HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. But contrary to other member of the ERBB family, HER2 does not directly bind ligand. HER2 activation results from heterodimerization with another ERBB member or by homodimerization when HER2 concentration are high, for instance in cancer. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.
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Immunofluorescence
