ICC shows positive staining in HT-29 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-EpCAM (PE Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
EpCAM Recombinant Rabbit mAb (PE Conjugate) (S-078-128)
EpCAM Recombinant Rabbit mAb (PE Conjugate) (S-078-128)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | EpCAM |
| Synonyms | Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein (EGP); Epithelial glycoprotein 314 (EGP314; hEGP314); CD326; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; EPCAM |
| Immunogen | Synthetic Peptide |
| Location | Cell junction, Lateral cell membrane |
| Accession | P16422 |
| Clone Number | S-078-128 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:200 | Hu |
| IF | 1:200-1:500 | Hu, Ms, Rt |
Background
EpCAM (epithelial cell adhesion molecule, CD326) is a 35–40 kDa type-I transmembrane glycoprotein expressed on most epithelia, where it first assembles into cis-dimers that further cluster into tetramers to support homotypic cell–cell contacts, and then—through sequential proteolytic cleavage—releases its intracellular domain (EpICD) that partners with β-catenin, FHL2 and LEF1 to enter the nucleus and drive Wnt-dependent transcription of genes controlling proliferation, stemness and epithelial-to-mesenchymal transition, thereby functioning not merely as an adhesion molecule but as a dynamic signaling hub that is highly up-regulated in most carcinomas, marks circulating tumor and cancer stem cells, and serves as both a prognostic biomarker and a therapeutic target while mutations that truncate or mis-localize the protein underlie the severe congenital diarrhea disorder tufting enteropathy.
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Immunocytochemistry
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon cancer. Anti-EpCAM (PE Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti-EpCAM (PE Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti-EpCAM (PE Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-EpCAM (PE Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat colon. Anti-EpCAM (PE Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
