ENO1 Mouse mAb
ENO1 Mouse mAb
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | ENO1 |
| Synonyms | Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; ENO1L1; MBPB1; MPB1; ENO1 |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P06733 |
| Antibody Type | Mouse mAb |
| Isotype | IgM |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, PC-3, Jurkat, mouse brain, mouse liver, rat brain, rat liver |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | Hu, Ms, Rt |
Background
ENO1 (Alpha-enolase) is a ubiquitous glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Beyond its canonical metabolic role, ENO1 functions as a multifunctional "moonlighting" protein: when expressed on the cell surface, it acts as a plasminogen receptor involved in fibrinolysis, cell migration, and tumor invasion. Clinically, ENO1 is frequently overexpressed in various cancers and serves as a valuable biomarker for tumor progression and autoimmune diseases.
Picture
Picture
Western Blot
WB result of ENO1 Mouse mAb
Primary antibody: ENO1 Mouse mAb at 1/5000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
WB result of ENO1 Mouse mAb
Primary antibody: ENO1 Mouse mAb at 1/5000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
WB result of ENO1 Mouse mAb
Primary antibody: ENO1 Mouse mAb at 1/5000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
