WB result of eIF6 Recombinant Rabbit mAb
Primary antibody:eIF6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K-562 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 24 kDa
eIF6 Recombinant Rabbit mAb (S-4171-13)
eIF6 Recombinant Rabbit mAb (S-4171-13)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eIF6 |
| Synonyms | Eukaryotic translation initiation factor 6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP); EIF3A; ITGB4BP; EIF6 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P56537 |
| Clone Number | S-4171-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, K562, HEK-293, HepG2, HCT 116, C2C12, RAW264.7 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:20000 | Hu, Ms |
| IHC-P | 1:2000 | Hu |
Background
The eukaryotic initiation factor 6 (eIF6) is a highly conserved translational regulatory protein found in eukaryotes and archaea, functioning primarily as an "anti-association" factor. It specifically binds to the 60S large ribosomal subunit, preventing its premature association with the 40S small subunit, thereby regulating the formation of functional 80S ribosomes. eIF6 exhibits dual functionality: within the nucleus, it participates in the maturation and nuclear export of the 60S large subunit, while in the cytoplasm, it interacts with the protein kinase C receptor RACK1 in response to cellular signals (such as insulin stimulation or PKC activation). Upon phosphorylation, eIF6 is released from the 60S subunit, thereby initiating protein translation. The protein possesses a unique five-fold symmetric β/α propeller structure. Dysregulation of eIF6 function affects cell growth and is implicated in cancer progression; recent studies have also revealed its involvement in regulating histone acetylation, thereby contributing to muscle metabolism and functional regulation.
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Picture
Western Blot
WB result of eIF6 Recombinant Rabbit mAb
Primary antibody:eIF6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: RAW 264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 24 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-eIF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-eIF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-eIF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-eIF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
