EIF4A3 Mouse mAb
EIF4A3 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | EIF4A3 |
| Synonyms | Eukaryotic initiation factor 4A-III; eIF-4A-III; eIF4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; Nuclear matrix protein 265 (NMP 265; hNMP 265); DDX48; KIAA0111 |
| Location | Cytoplasm, Nucleus |
| Accession | P38919 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, HepG2, Jurkat, K-562, NIH/3T3, 4T1, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:2000-1:5000 | Hu, Ms, Rt |
Background
EIF4A3 (eukaryotic initiation factor 4A3) is a member of the DEAD-box RNA helicase family and an ATP-dependent RNA helicase that serves as the core scaffold protein of the exon junction complex (EJC). It adopts a butterfly-shaped conformation through its N-terminal and C-terminal RecA-like domains, which are used to tightly assemble with other core EJC components (such as Y14, Magoh, and MLN51) into a stable heterotetrameric core complex. The EJC is deposited approximately 20–24 nucleotides upstream of mRNA exon-exon junctions during splicing, where EIF4A3 binds and locks specific structures on spliced mRNAs to ensure stable assembly, thereby playing crucial roles in multiple post-transcriptional regulatory processes, including mRNA export, nonsense-mediated mRNA decay (NMD), translation efficiency, and localization. Dysfunction of EIF4A3 is associated with its aberrant expression in various cancers: in hepatocellular carcinoma, high expression of EIF4A3 promotes cell proliferation and metastasis; in glioblastoma, upregulated EIF4A3 expression is also correlated with poor patient prognosis and can influence the tumor microenvironment and immune responses through regulation of NMD pathways of target genes. Furthermore, loss-of-function mutations in EIF4A3 cause a rare autosomal recessive disorder known as Richieri-Costa-Pereira syndrome, which is primarily characterized by severe distal limb malformations, cleft palate, and intellectual developmental delay.
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Western Blot
WB result of EIF4A3 Mouse mAb
Primary antibody: EIF4A3 Mouse mAb at 1/25000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
WB result of EIF4A3 Mouse mAb
Primary antibody: EIF4A3 Mouse mAb at 1/25000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 4T1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
WB result of EIF4A3 Mouse mAb
Primary antibody: EIF4A3 Mouse mAb at 1/25000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-EIF4A3 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-EIF4A3 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-EIF4A3 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-EIF4A3 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-EIF4A3 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
