WB result of eIF3G Rabbit pAb
Primary antibody: eIF3G Rabbit pAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 40 kDa
eIF3G Rabbit Polyclonal Antibody
eIF3G Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eIF3G |
| Synonyms | Eukaryotic translation initiation factor 3 subunit G; Eukaryotic translation initiation factor 3 RNA-binding subunit (eIF-3 RNA-binding subunit); Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44; EIF3S4 UniRule Annotation; EIF3G |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O75821 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Mk |
| Positive Sample | Raji, HL-60, K562, HepG2, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:250 | Hu |
Background
eIF3G, also known as eukaryotic translation initiation factor 3 subunit G or p35, is a crucial component of the eIF3 multi-protein complex, which serves as the largest and most complex initiation factor in eukaryotic translation. Structurally, eIF3G contains multiple RNA recognition motifs (RRMs) that enable it to directly bind specific mRNA sequences and interact with other eIF3 subunits, thereby playing a pivotal role in stabilizing the eIF3 complex and facilitating the assembly of the 43S pre-initiation complex at the 5' cap of messenger RNAs. Beyond its canonical function in promoting the recruitment of the 40S ribosomal subunit to mRNA, eIF3G has been implicated in the selective translation of specific transcripts involved in cell cycle regulation and stress responses, and its dysregulation or overexpression has been frequently observed in various human cancers, suggesting its potential as a biomarker and therapeutic target in oncology due to its influence on protein synthesis rates and cellular proliferation.
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Western Blot
WB result of eIF3G Rabbit pAb
Primary antibody: eIF3G Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 40 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Eif3g antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Eif3g antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Eif3g antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
