WB result of EIF2S2 Rabbit Polyclonal Antibody
Primary antibody: EIF2S2 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 48 kDa
EIF2S2 Rabbit Polyclonal Antibody
EIF2S2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | EIF2S2 |
| Synonyms | Eukaryotic translation initiation factor 2 subunit 2; EIF2B |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P20042 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, Jurkat, HepG2, NIH/3T3, mouse liver, mouse testis, C6, PC-12, rat liver, COS-7 |
| Predicted Reactivity | Bv, Rb |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
EIF2S2 (eukaryotic translation initiation factor 2 subunit 2, also known as eIF2-β) is the β subunit of the heterotrimeric eIF2 complex, composed of 333 amino acids with a molecular weight of approximately 38 kDa, and it plays a key role in the initiation phase of protein synthesis. The eIF2 complex forms a ternary complex with GTP and initiator tRNA, binds the 40S ribosomal subunit, and subsequently recruits mRNA to assemble the 43S preinitiation complex. Structurally, EIF2S2 contains a highly charged N-terminal region (harboring multiple polylysine blocks), a zinc finger motif, and a C-terminal eIF2-β/eIF-5 homology domain, features that together confer mRNA-binding capability. Functionally, eIF2-β participates in regulating the activity of the eIF2 complex and influences the accuracy of translation initiation and the recycling of eIF2 through interactions with factors such as eIF5. Recent studies have found that EIF2S2 is highly expressed in a variety of tumors, including breast cancer, hepatocellular carcinoma, and glioblastoma, and is associated with tumor aggressiveness and poor prognosis; by regulating selective translation, it affects the expression of downstream genes such as ATF4 and CHOP, thereby participating in the regulation of cellular stress responses, proliferation, and apoptosis.
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Western Blot
WB result of EIF2S2 Rabbit Polyclonal Antibody
Primary antibody: EIF2S2 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 48 kDa
WB result of EIF2S2 Rabbit Polyclonal Antibody
Primary antibody: EIF2S2 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 48 kDa
WB result of EIF2S2 Rabbit Polyclonal Antibody
Primary antibody: EIF2S2 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 48 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-EIF2S2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-EIF2S2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-EIF2S2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-EIF2S2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-EIF2S2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-EIF2S2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
