WB result of EIF2B5 Recombinant Rabbit mAb
Primary antibody: EIF2B5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
EIF2B5 Recombinant Rabbit mAb (S-3816-3)
EIF2B5 Recombinant Rabbit mAb (S-3816-3)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | EIF2B5 |
| Synonyms | Translation initiation factor eIF2B subunit epsilon; EIF2BE |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q13144 |
| Clone Number | S-3816-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Raji, HT-1080, A549, HT-29, HeLa, NIH/3T3, mouse brain, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:400 | Hu |
Background
EIF2B5 is a core regulatory factor that maintains the balance between protein synthesis and cellular stress responses in eukaryotes. As the largest catalytic subunit (ε subunit) of the EIF2B complex—a guanine nucleotide exchange factor composed of five different subunits (α to ε)—EIF2B5 is responsible for catalyzing the key reaction of removing GDP from the translation initiation factor EIF2 and replacing it with GTP, thereby activating EIF2 and initiating mRNA translation. Under conditions such as endoplasmic reticulum stress, nutrient deprivation, or viral infection, the EIF2α subunit becomes phosphorylated, which then binds to and inhibits the EIF2B complex with high affinity, forming a stable "suicidal" complex that leads to global downregulation of protein synthesis to conserve energy and promote stress recovery. Recessive mutations in the EIF2B5 gene are among the five causative genes for vanishing white matter disease, a condition characterized by progressive motor deterioration and cognitive impairment, with MRI showing diffuse leukoencephalopathy with cystic degeneration. Furthermore, as a core node in the integrated stress response (ISR) pathway, dysregulation of EIF2B5 is closely associated with various pathological states including neurodegenerative diseases, cancer, and viral infections, making it a potential target for therapeutic intervention.
Picture
Picture
Western Blot
WB result of EIF2B5 Recombinant Rabbit mAb
Primary antibody: EIF2B5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
WB result of EIF2B5 Recombinant Rabbit mAb
Primary antibody: EIF2B5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
Immunocytochemistry
ICC shows positive staining in Raji cells. Anti- EIF2B5 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
