WB result of eIF2α Rabbit pAb
Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 37 kDa
eIF2α Rabbit Polyclonal Antibody
eIF2α Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eIF2α |
| Synonyms | Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha; eIF-2A; eIF-2alpha; eIF2-alpha); EIF2A; EIF2S1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Mitochondrion |
| Accession | P05198 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | PC-3, MCF7, Ramos, HepG2, NIH/3T3, PC-12, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
eIF2α (eukaryotic initiation factor 2 subunit alpha) is a 35 kDa phosphoprotein that, together with the G-protein βγ dimer eIF2B, forms the heterotrimeric eIF2 complex essential for delivering the initiator methionyl-tRNA to the 40S ribosomal subunit in a GTP-dependent manner; phosphorylation of Ser51 by stress-sensing kinases (PKR, PERK, HRI, GCN2) converts eIF2 into a non-competitive inhibitor of its guanine-nucleotide-exchange factor eIF2B, thereby repressing global cap-dependent translation while selectively permitting ATF4 and other stress-response mRNAs, a switch that underlies cellular adaptation to starvation, oxidative stress, viral infection, and proteotoxicity, and whose dysregulation contributes to diseases ranging from neurodegeneration and cancer to diabetes and viral pathogenesis.
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Picture
Western Blot
WB result of eIF2α Rabbit pAb
Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 37 kDa
WB result of eIF2α Rabbit pAb
Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 37 kDa
WB result of eIF2α Rabbit pAb
Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 37 kDa
