The EGFR[T790M/C797S/L858R] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[T790M/C797S/L858R] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P00533-1 |
| Amino Acid Sequence | R669-A1210(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 87855.0 |
| Purity | >85% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Hepes, 150mM NaCl, 20mM Reduced Glu, 1mM DTT, 10% Glycerol, pH7.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity


SDS-PAGE

M MW Standard
1 1 μg EGFR[T790M/C797S/L858R]
2 3 μg EGFR[T790M/C797S/L858R]
