| Species | Human |
| Synonyms | EGFR(d746-750/T790M/C797S) |
| Accession | P00533-1 |
| Amino Acid Sequence |
R669-A1210(end), d746-750, T790M, C797S
RRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKTSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGSLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
|
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 87.2 kDa |
| Purity | > 90% by SDS-PAGE |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Hepes, 150 mM NaCl, 1 mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5 |
| Stability & Storage |
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
EGFR[d746-750/T790M/C797S] titration and inhibitor IC50 determination
The EGFR[d746-750/T790M/C797S] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[d746-750/T790M/C797S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
M MW Standard
1 1 μg EGFR[d746-750/T790M/C797S]
2 3 μg EGFR[d746-750/T790M/C797S]
