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EGFR Fc Chimera Protein, Human

EGFR Fc Chimera Protein, Human

Catalog Number: UA010174 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE
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Regular price $648.00 SGD
Regular price Sale price $648.00 SGD
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Product Details

Product Specification


Species Human
Synonyms ERBB,ERRP,HER1,Mena,ERBB1
Accession P00533-1
Amino Acid Sequence

Leu25-Ser645, with C-terminal Human IgG1 Fc

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

Expression System HEK293
Molecular Weight 100-125kDa (Reducing)
Purity

>95% by SDS-PAGE

Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag Human Fc Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

Background

The Epidermal Growth Factor Receptor (EGFR)is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

EGFR is activated after homodimerization or heterodimerization. Heterodimerization is an important mechanism that allows the activation of all EGFR in response to ligand stimulation. The activated EGFR binds to many signaling proteins and stimulates the activation of many signaling pathways. The specificity and potency of intracellular signaling pathways are determined by positive and negative regulators, the specific composition of activating ligand(s), receptor dimer components, and the diverse range of proteins that associate with the tyrosine phosphorylated C-terminal domain of the EGFR.EGFR is overexpressed or mutated in many cancers, especially in breast cancer, ovarian cancer, and non-small cell lung cancer. The overexpression and overactivation of EGFR is correlated with poor prognosis, drug resistance, cancer metastasis, and lower survival rate.

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Bioactivity

EGFR Fc Chimera, Human (Cat. No. UA010174) captured on Protein A Biosenor, can bind HB-EGF, Human (Cat. No. UA040029) with an affinity constant of 0.39 μM as determined in SPR assay.
Immobilized EGF, Human (Cat. No. UA040003) at 0.5μg/mL (100μL/well) can bind EGFR Fc Chimera, Human (Cat. No. UA010174) with EC50 of 0.11-0.15μg/ml.
CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR Fc Chimera, Human (Cat. No. UA010174) with an affinity constant of 0.31nM as determined in SPR assay.

SDS-PAGE

1μg (R: reducing conditions, N: non-reducing conditions).

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