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EGF Protein, Human

EGF Protein, Human

Catalog Number: UA040575 Brand: UA BIOSCIENCE
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$25 USD
$25 USD
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Product Details

Product Specification


Species Human
Synonyms Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone
Accession P01133
Amino Acid Sequence

Asn971-Arg1023
Expression System E.coli
Molecular Weight

10 kDa (Reducing)
Purity >95% by SDS-PAGE & HPLC.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.
Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-6

Background

Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Epidermal growth factor (EGF) binds to epidermal growth factor receptor and stimulates an intracellular signal transduction cascade, leading to activation of genes that regulate cell proliferation, angiogenesis, motility, and metastasis. Epidermal growth factor (EGF) mRNA and protein are expressed in a number of adult tissues, especially in epithelial cells in the gastrointestinal tract. Predominant sites of synthesis of this peptide are the submandibular glands, the Brunner glands in the small intestine and the kidney.

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Bioactivity

Measured in a cell inhibition assay using A431 cell. The expected IC50 is 2.3 ng/ml.

EGF Protein, Human (Cat#UA040575) maintains colorectal cancer organoid growth well during multiple passages and long-term culture processes.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

>95% as determined by SEC-HPLC.

RP-HPLC

>95% as determined by RP-HPLC.