| Species | Human |
| Accession | P01133 |
| Amino Acid Sequence |
Asn971-Arg1023, with an N-terminal Met MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIGERCQYRDLKW WELR |
| Expression System | E.coli |
| Molecular Weight | 7kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.2EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Epidermal growth factor (EGF) binds to epidermal growth factor receptor and stimulates an intracellular signal transduction cascade, leading to activation of genes that regulate cell proliferation, angiogenesis, motility, and metastasis. Epidermal growth factor (EGF) mRNA and protein are expressed in a number of adult tissues, especially in epithelial cells in the gastrointestinal tract. Predominant sites of synthesis of this peptide are the submandibular glands, the Brunner glands in the small intestine and the kidney.
Primary antibody: Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb(S0B0851) at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 100 ng/ml EGF(UA040003) for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 90 kDa
Immobilized EGF, Human (Cat. No. UA040003) at 0.5μg/mL (100μL/well) can bind EGFR Fc Chimera, Human (Cat. No. UA010174) with EC50 of 0.11-0.15μg/ml.
Immobilized EGF, Human (Cat. No. UA040003) at 1.0μg/mL (100μL/well) can bind EGFR His Tag, Rhesus macaque (Cat. No. UA010168) with EC50 of 0.54-1.0μg/ ml.
Immobilized EGF, Human (Cat. No. UA040003) at 1.5μg/mL (100μL/well) can bind EGFR His Tag, Mouse (Cat. No. UA010161) with EC50 of 0.84-1.44μg/ ml.
CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Human (Cat. No. UA010306) with an affinity constant of 2.01μM as determined in SPR assay.
CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Rhesus macaque (Cat. No. UA010168) with an affinity constant of 0.53mM as determined in SPR assay.
