WB result of eEF2K Rabbit pAb
Primary antibody: eEF2K Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 100 kDa
eEF2K Rabbit Polyclonal Antibody
eEF2K Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eEF2K |
| Synonyms | Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Synapse |
| Accession | O00418 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | HEK-293, 293T, HeLa, K562, PC-3, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| ICC | 1:500 | Hu |
Background
eEF2K (eukaryotic elongation factor 2 kinase) is a highly conserved Ca²⁺/calmodulin-dependent serine/threonine kinase that acts as a critical metabolic checkpoint by phosphorylating and inactivating eukaryotic elongation factor 2 (eEF2) at Thr56, thereby shutting down the energy-intensive translocation step of ribosomal translation elongation to conserve ATP during nutrient starvation, hypoxia, or oxidative stress; beyond this canonical translational control, eEF2K also exerts non-canonical, kinase- or eEF2-independent functions—modulating transcription factors such as p53, promoting DNA-damage repair, maintaining mitochondrial quality, and enhancing tumor cell survival in nutrient-poor microenvironments—while its own activity is tightly regulated by multiple upstream signaling pathways including mTORC1-mediated inhibition and AMPK- or TRPM7-mediated activation, making it a potential biomarker and therapeutic target in cancer and metabolic diseases.
Picture
Picture
Western Blot
WB result of eEF2K Rabbit pAb
Primary antibody: eEF2K Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 100 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- eEF2K antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
