WB result of eEF2 Recombinant Rabbit mAb
Primary antibody: eEF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 110 kDa
eEF2 Recombinant Rabbit mAb (S-1976-35)
eEF2 Recombinant Rabbit mAb (S-1976-35)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eEF2 |
| Synonyms | Elongation factor 2; EF-2; EF2; EEF2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P13639 |
| Clone Number | S-1976-35 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, A431, HEK-293, NIH/3T3, C6, human breast cancer, human cervix cancer, human stomach cancer, human colon, human liver |
| Predicted Reactivity | Zf, Ck, Bv, Mrst, Hm |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
Background
Eukaryotic elongation factor 2 (eEF2) is a crucial regulator of protein synthesis, belonging to the highly conserved guanosine triphosphatase (GTPase) family. It is essential for the elongation phase of protein translation, facilitating the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. eEF2's activity is tightly regulated by phosphorylation at Thr56 by eEF2 kinase (eEF2K), which inhibits its function and thus suppresses protein synthesis. This protein is ubiquitously expressed in various tissues and plays a significant role in normal cellular functions. However, its dysregulation is implicated in several diseases, including cancer, where overexpression of eEF2 is associated with increased tumor incidence and poorer patient prognosis.
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Picture
Western Blot
WB result of eEF2 Recombinant Rabbit mAb
Primary antibody: eEF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 110 kDa
WB result of eEF2 Recombinant Rabbit mAb
Primary antibody: eEF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 110 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling eEF2 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HEK-293 cells. Anti- eEF2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression Atlas
Expression of eEF2 in tumor tissue.
Expression of eEF2 in human tissue.
Expression of eEF2 in mouse and rat tissue.
