EBV Induced Gene 2/EBI2 Recombinant Rabbit mAb (S-5946)

Catalog Number: S0B60492 Application: IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	EBV Induced Gene 2/EBI2
Synonyms	G-protein coupled receptor 183; Epstein-Barr virus-induced G-protein coupled receptor 2 (EBI2; EBV-induced G-protein coupled receptor 2; hEBI2); EBI2; GPR183
Location	Cell membrane
Accession	P32249
Clone Number	S-5946
Antibody Type	Recombinant mAb
Isotype	IgG
Application	IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:50-1:100	Hu

Background

EBV-induced gene 2 (EBI2), also known as GPR183, is a G protein-coupled receptor initially identified as being significantly upregulated in EBV-infected Burkitt's lymphoma cell lines, and is predominantly highly expressed on immune cells such as B cells. Functionally, the most central role of EBI2 is as a chemotactic receptor, guiding B cells to precisely migrate to extrafollicular regions and bridging channels within secondary lymphoid organs such as the spleen, in response to concentration gradients of its endogenous ligands—oxysterols such as 7α,25-dihydroxycholesterol (7α,25-OHC). This process acts synergistically with signaling from the chemokine receptors CXCR5 and CCR7 to collectively orchestrate the spatial positioning of T-cell-dependent antibody responses. Beyond this classical function in B cell localization, EBI2 also plays important roles in broader immunoregulation: under inflammatory conditions, stromal cells such as those in high endothelial venules within lymph nodes upregulate the expression of ligand-synthesizing enzymes like cholesterol 25-hydroxylase (CH25H), making EBI2 signaling a critical pathway for recruiting naïve lymphocytes into inflamed lymph nodes. Simultaneously, the EBI2 signaling axis is also involved in the regulation of myelination and remyelination, where its loss of function or antagonism impairs this process, positioning it as a potential therapeutic target for autoimmune diseases of the central nervous system such as multiple sclerosis. This multifaceted nature of EBI2 renders it a pivotal hub molecule connecting infection, immune responses, and autoimmune diseases, and it is regarded as a highly promising drug target.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-EBV Induced Gene 2/EBI2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-EBV Induced Gene 2/EBI2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-EBV Induced Gene 2/EBI2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human brain. Anti-EBV Induced Gene 2/EBI2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-EBV Induced Gene 2/EBI2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.