WB result of E tag Recombinant Rabbit mAb
Primary antibody: E tag Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: E tag protein 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 26 kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | E tag |
Immunogen | Synthetic Peptide |
Clone Number | S-911-14 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | WB, ICC, IP |
Reactivity | Species Independent |
Purification | Protein A |
Concentration | 0.5 mg/ml |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
application | dilution | species |
WB | 1:5000 | |
IP | 1:50 | |
ICC | 1:500 |
Background
The E tag is an epitope tag with a 13-residue sequence of GAPVPYPDPLEPR. The E tag can help with detection of proteins in western blot, IP, or microscopic techniques.
Picture
Picture
Western Blot
IP
E tag Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating E tag in 0.4 mg 293T transfected with Histone H3-mCherry-E-tag whole cell lysate.
Western blot was performed on the immunoprecipitate using E tag Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: 293T transfected with Histone H3-mCherry-E-tag whole cell lysate 5 µg (Input)
Lane 2: E tag Rabbit mAb IP in 293T transfected with Histone H3-mCherry-E-tag whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in 293T transfected with Histone H3-mCherry-E-tag whole cell lysate
Predicted MW: 26 kDa
Observed MW: 26,50 kDa
