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E tag Recombinant Rabbit mAb (S-911-14)

E tag Recombinant Rabbit mAb (S-911-14)

Catalog Number: S0B0759 Application: WB,ICC,IP Reactivity: Other Conjugation: Unconjugated Brand: Starter
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Regular price $45 USD
Regular price Sale price $45 USD
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Product Details

Product Specification


Host Rabbit
Antigen E tag
Immunogen Synthetic Peptide
Clone Number S-911-14
Antibody Type Recombinant mAb
Isotype IgG
Application WB, ICC, IP
Reactivity Species Independent
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage 12 months from date of receipt / reconstitution, -20 °C as supplied.

Dilution


application dilution species
WB 1:5000
IP 1:50
ICC 1:500

Background

The E tag is an epitope tag with a 13-residue sequence of GAPVPYPDPLEPR. The E tag can help with detection of proteins in western blot, IP, or microscopic techniques.

Picture

Western Blot

WB result of E tag Recombinant Rabbit mAb
Primary antibody: E tag Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: E tag protein 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 26 kDa

IP

E tag Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating E tag in 0.4 mg 293T transfected with Histone H3-mCherry-E-tag whole cell lysate.
Western blot was performed on the immunoprecipitate using E tag Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: 293T transfected with Histone H3-mCherry-E-tag whole cell lysate 5 µg (Input)
Lane 2: E tag Rabbit mAb IP in 293T transfected with Histone H3-mCherry-E-tag whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in 293T transfected with Histone H3-mCherry-E-tag whole cell lysate
Predicted MW: 26 kDa
Observed MW: 26,50 kDa

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