WB result of E-selectin Recombinant Rabbit mAb
Primary antibody: E-selectin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HUVEC whole cell lysate 20 µg
Lane 2: HUVEC treated with 25 ng/ml TNF-alpha for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 90~120 kDa
E-selectin Recombinant Rabbit mAb (S-1260-26)
E-selectin Recombinant Rabbit mAb (S-1260-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | E-selectin |
| Synonyms | CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1 (ELAM-1), Leukocyte-endothelial cell adhesion molecule 2 (LECAM2), CD62E, SELE, ELAM1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16581 |
| Clone Number | S-1260-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:500 | null |
Background
E-selectin, also known as CD62E, is a cell adhesion molecule that plays a significant role in the immune system, particularly during inflammation and the recruitment of leukocytes to sites of infection or tissue damage. It is expressed on the surface of endothelial cells and facilitates the rolling and adhesion of leukocytes, such as neutrophils and monocytes, along the endothelium. E-selectin's interaction with its ligands on leukocytes is crucial for the initiation of inflammatory responses. It binds to specific carbohydrate structures present on the surface of leukocytes, mediating the initial steps of leukocyte extravasation into tissues. This process is highly regulated and involves a dynamic expression of E-selectin, which is upregulated in response to inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). In addition to its role in leukocyte recruitment, E-selectin has been implicated in the activation of cellular processes within neutrophils.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- E-selectin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- E-selectin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
