1μg (R: reducing condition, N: non-reducing condition).
DT3C (Diphtheria toxin & spg 3C domain) Protein, Corynephage beta
DT3C (Diphtheria toxin & spg 3C domain) Protein, Corynephage beta
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$240.00 USD
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Product Details
Product Details
Product Specification
| Synonyms | DT3C, Diphtheria toxin & spg 3C domain |
| Accession | P00588、P19909 |
| Amino Acid Sequence | Gly33-Lys417&&ILe291-Glu497 |
| Expression System | E.coli |
| Molecular Weight | 65kDa (Reducing) |
| Purity | ＞90% by SDS-PAGE and HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, -20 to -70 °C as supplied. |
| Reference | Biochem Biophys Res Commun. 2014 Nov 28; 454(4):600-3.Epub 2014 Nov 1. |
Background
DT3C consists of diphtheria toxin (DT) lacking the receptor-binding domain but containing the C1, C2, and C3 domains of Streptococcus protein G (3C). When a mAb–DT3C conjugate, which functions in vitro like an ADC, reduces the viability of cancer cells, the mAb being tested must have been internalized by the target cells. DT3C can thus be a tool to identify efficiently and easily mAbs that can be internalized by cells, thereby enhancing the development of promising ADCs.
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Bioactivity
SDS-PAGE
RP-HPLC
>90% as determined by RP-HPLC.
