WB result of DPEP1 Recombinant Rabbit mAb
Primary antibody: DPEP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: SW620 whole cell lysate 20 µg
Lane 4: COLO 205 whole cell lysate 20 µg
Negative control: SW480 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 46 kDa
Observed MW: 55 kDa
DPEP1 Recombinant Rabbit mAb (S-740-249)
DPEP1 Recombinant Rabbit mAb (S-740-249)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DPEP1 |
| Synonyms | Dipeptidase 1; Beta-lactamase; Dehydropeptidase-I; Microsomal dipeptidase; Renal dipeptidase (hRDP); MDP; RDP |
| Immunogen | Synthetic Peptide |
| Location | Cell projection, Apical cell membrane |
| Accession | P16444 |
| Clone Number | S-740-249 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | HepG2, SW620, COLO 205 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Dipeptidase 1 (DPEP1), or renal dipeptidase, is a membrane-bound glycoprotein responsible for hydrolyzing dipeptides. It is found in the microsomal fraction of the porcine kidney cortex. It exists as a disulfide-linked homodimer that is glygosylphosphatidylinositol (GPI)-anchored to the renal brush border of the kidney. The primary function of DPEP1 is to hydrolyze various dipeptides in renal metabolism. Specifically, it has been found to hydrolyze glutathione and its conjugates such as leukotriene D.
Picture
Picture
Western Blot
