WB result of DOCK11 Recombinant Rabbit mAb
Primary antibody: DOCK11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 238 kDa
Observed MW: 250 kDa
DOCK11 Recombinant Rabbit mAb (S-4420-57)
DOCK11 Recombinant Rabbit mAb (S-4420-57)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DOCK11 |
| Synonyms | Dedicator of cytokinesis protein 11; Activated Cdc42-associated guanine nucleotide exchange factor (ACG); Zizimin-2; ZIZ2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q5JSL3 |
| Clone Number | S-4420-57 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, K-562, Raw 264.7, NIH/3T3, mouse testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:500 | Hu, Ms |
| ICC | 1:100 | Hu |
| IF | 1:500 | Ms |
Background
DOCK11, also known as Zizimin2, is an atypical guanine nucleotide exchange factor (GEF) encoded by the DOCK11 gene. It belongs to class D of the DOCK protein family and possesses a unique structure that lacks the conventional Dbl homology (DH) domain; instead, it exerts its catalytic function through its C-terminal DHR-2 domain, specifically activating the small GTPase CDC42. Functionally, DOCK11 is predominantly expressed in hematopoietic cells and lymphocytes, where it regulates dynamic actin cytoskeleton reorganization by activating CDC42, thereby promoting filopodia formation and influencing lymphocyte polarization and migration. This function is critically important in the immune system; studies have confirmed that loss-of-function mutations in the DOCK11 gene cause an X-linked early-onset multisystem autoinflammatory disease characterized by immune dysregulation, autoimmune cytopenias, and systemic lupus erythematosus. Lymphocytes from patients with this disease exhibit reduced CDC42 activity, impaired migration, and excessive production of pro-inflammatory cytokines due to DOCK11 deficiency.
Picture
Picture
Western Blot
WB result of DOCK11 Recombinant Rabbit mAb
Primary antibody: DOCK11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raw 264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 238 kDa
Observed MW: 250 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-DOCK11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DOCK11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-DOCK11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-DOCK11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti- DOCK11 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse testis. Anti-DOCK11 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
