WB result of DNMT3A Recombinant Rabbit mAb
Primary antibody: DNMT3A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NCCIT whole cell lysate 20 µg
Lane 2: NETRA-2 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 85, 95, 120 kDa
DNMT3A Recombinant Rabbit mAb (S-4120-54)
DNMT3A Recombinant Rabbit mAb (S-4120-54)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DNMT3A |
| Synonyms | DNA (cytosine-5)-methyltransferase 3A; Cysteine methyltransferase DNMT3A; DNA methyltransferase HsaIIIA (DNA MTase HsaIIIA; M.HsaIIIA) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9Y6K1 |
| Clone Number | S-4120-54 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | NCCIT, NETRA-2, HCT 116, 293T, NIH/3T3, F9, mouse brain, C6, rat brain, COS-7 |
| Predicted Reactivity | Ck |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
DNMT3A (DNA methyltransferase 3 alpha) is a crucial de novo DNA methyltransferase enzyme in mammals that establishes new cytosine methylation patterns, primarily at CpG dinucleotides, during early embryonic development and germ cell formation, distinct from DNMT1 which maintains existing methylation marks. Structurally, it contains a catalytic C-terminal domain and an N-terminal regulatory region with ADD and PWWP domains that facilitate interaction with histone tails and chromatin, thereby guiding precise genomic targeting. Beyond its canonical role in epigenetic silencing and regulation of gene expression, DNMT3A is vital for cellular differentiation, genomic imprinting, and X-chromosome inactivation; notably, somatic mutations in the DNMT3A gene, particularly at residue R882, are frequently observed in acute myeloid leukemia (AML) and other hematologic malignancies, as well as in overgrowth syndromes like Tatton-Brown-Rahman syndrome, highlighting its dual significance as a key regulator of normal development and a critical tumor suppressor whose dysfunction leads to aberrant hypomethylation and oncogenic transformation.
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Western Blot
WB result of DNMT3A Recombinant Rabbit mAb
Primary antibody: DNMT3A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: F9 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 85, 95, 120 kDa
This blot was developed with high sensitivity substrate
WB result of DNMT3A Recombinant Rabbit mAb
Primary antibody: DNMT3A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 95, 120 kDa
This blot was developed with high sensitivity substrate
WB result of DNMT3A Recombinant Rabbit mAb
Primary antibody: DNMT3A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 95, 120 kDa
This blot was developed with high sensitivity substrate
