1ug (R: reducing conditions, N: non-reducing conditions).
DNAM-1/CD226 Fc Chimera Protein, Human
DNAM-1/CD226 Fc Chimera Protein, Human
Price:
Regular price
$460.00 USD
Regular price
Sale price
$460.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | DNAM-1/CD226 |
| Synonyms | PTA1 |
| Accession | Q15762 |
| Amino Acid Sequence | Glu19-Asn247, with C-terminal Human IgG1 Fc |
| Expression System | HEK293 |
| Molecular Weight | 70-75kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. WANG, MENGJIE, BU, JIN, ZHOU, MAOHUA, et al. CD8(+) T cells expressing both PD-1 and TIGIT but not CD226 are dysfunctional in acute myeloid leukemia (AML) patients[J]. Clinical immunology: The official journal of the Clinical Immunology Society,2018,19064-73. |
Background
DNAM-1 is a member of the Ig superfamily, encoded by a gene on human chromosome 18q22.3, is expressed by human NK cells, T cells, a subset of B cells, monocytes and platelets. Interactions between DNAM-1 on NK cells and its ligands on tumour cells augments the NK cell–mediated cytotoxicity and cytokine production. CD155 and its related family member CD112, the known ligands for DNAM-1, broadly expressed on hematopoietic, epithelial, and endothelial cells. Moreover, it was shown that the interaction of DNAM-1 with CD112 and CD155 contributes to the NK-mediated lysis of both imDCs and mDCs. Dysregulation of DNAM-1 is associated with susceptibility to juvenile idiopathic arthritis, type 1 diabetes (T1D), lupus, and rheumatoid arthritis in patients.
Picture
Picture
SDS-PAGE
ELISA
Immobilized CD155/PVR His Tag, Human (Cat. No. UA010069) at 2.0μg/mL (100μL/well) can bind DNAM-1/CD226 Fc Chimera, Human (Cat. No. UA010560) with EC50 of 4.48-13.43μg/ml.
SPR
Protein A Chip captured DNAM-1/CD226 Fc Chimera, Human(Cat. No. UA010560), can bind CD155/PVR His Tag, Human(Cat. No. UA010069) with an affinity constant of 1.28μM as determined in SPR assay.
