WB result of DNA Ligase IV/LIG4 Recombinant Rabbit mAb
Primary antibody: DNA Ligase IV/LIG4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 104 kDa
Observed MW: 104 kDa
DNA Ligase IV/LIG4 Recombinant Rabbit mAb (S-2237-64)
DNA Ligase IV/LIG4 Recombinant Rabbit mAb (S-2237-64)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DNA Ligase IV/LIG4 |
| Synonyms | DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P49917 |
| Clone Number | S-2237-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Rt |
| Positive Sample | PC-12, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Rt |
| IHC-P | 1:200 | Rt |
Background
DNA Ligase IV, also known as LIG4, is a crucial enzyme involved in the non-homologous end joining (NHEJ) pathway, which is essential for repairing DNA double-strand breaks. It plays a vital role in maintaining genomic stability by facilitating the ligation of broken DNA ends. LIG4 forms a complex with other proteins such as XRCC4 and XLF, enhancing its stability and activity. This enzyme is particularly important in the repair of DNA damage caused by ionizing radiation and certain chemotherapeutic agents. Mutations or deficiencies in LIG4 can lead to severe immunodeficiency and developmental disorders due to impaired V(D)J recombination, a process critical for the generation of antigen receptor diversity in the immune system.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded rat colon. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-DNA Ligase IV/LIG4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
