WB result of DLDH Recombinant Rabbit mAb
Primary antibody: DLDH Recombinant Rabbit mAb at 1/3000 dilution
Lane 1: SK-OV-3 whole cell lysate 25 μg
Lane 2: K-562 whole cell lysate 25 μg
Lane 3: A-431 whole cell lysate 25 μg
Lane 4: BxPC-3 whole cell lysate 25 μg
Lane 5: HeLa whole cell lysate 25 μg
Lane 6: RD whole cell lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 55 kDa
DLDH Recombinant Rabbit mAb
DLDH Recombinant Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DLDH |
| Immunogen | Recombinant Protein |
| Location | Nucleus, Mitochondrion |
| Accession | P09622 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SK-OV-3, K-562, A-431, BxPC-3, HeLa, RD, NIH/3T3, C2C12, mouse liver, mouse heart, C6, rat liver, rat heart |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:3000-1:10000 | Hu, Ms, Rt |
| IHC-P | 1:400-1:1000 | Hu, Ms, Rt |
Background
DLDH (Dihydrolipoamide Dehydrogenase) is a mitochondrial flavin-dependent oxidoreductase encoded by the DLD gene; in humans, its classical function is to serve as the E3 component of the pyruvate dehydrogenase complex, the α-ketoglutarate dehydrogenase complex, and the branched-chain α-ketoacid dehydrogenase complex, and it is also a component of the glycine cleavage system, catalyzing the oxidation of dihydrolipoamide using NAD⁺ as the electron acceptor. The enzyme exists as a homodimer, with each subunit binding one molecule of FAD, and its active site contains a redox-active disulfide bond. Beyond its classical dehydrogenase function, DLDH also possesses diaphorase activity, being able to oxidize NADH using various electron acceptors such as O₂, iron ions, nitric oxide, or ubiquinone; under certain conditions (e.g., when its heterodimeric state is disrupted), it can also exhibit serine protease activity and participate in the cleavage of the mitochondrial iron chaperone frataxin. DLDH is sensitive to oxidative modifications, and defects in its function can cause the human autosomal recessive metabolic disease DLDD, manifesting as lactic acidosis and neurodegeneration. In addition, a portion of DLDH is also localized to the nucleus, where it participates in α-ketoglutarate dehydrogenase complex-dependent regulation of histone succinylation.
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Western Blot
WB result of DLDH Recombinant Rabbit mAb
Primary antibody: DLDH Recombinant Rabbit mAb at 1/3000 dilution
Lane 1: NIH/3T3 whole cell lysate 25 μg
Lane 2: C2C12 whole cell lysate 25 μg
Lane 3: mouse liver lysate 25 μg
Lane 4: mouse heart lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 55 kDa
WB result of DLDH Recombinant Rabbit mAb
Primary antibody: DLDH Recombinant Rabbit mAb at 1/3000 dilution
Lane 1: C6 whole cell lysate 25 μg
Lane 2: rat liver lysate 25 μg
Lane 3: rat heart lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-DLDH antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
