Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293T human embryonic kidney cells. Recombinant Mouse DKK-1 inhibits a constant dose of 50 ng/ml of Recombinant Human Wnt‑Surrogate. The EC50 for this effect is less than 90 ng/ml.
DKK-1 Protein, Mouse
DKK-1 Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Dickkopf-related protein 1; Dickkopf-1; Dkk-1; mDkk-1; |
| Accession | O54908 |
| Amino Acid Sequence | Ser30-His272, with N-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 38-70 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | N Engl J Med. 2003 Dec 25;349(26):2483-94. |
Background
Dickkopf-1 (DKK-1) is a secreted protein that belongs to the Dickkopf family of Wnt signaling inhibitors. In mice, DKK-1 is expressed in various tissues, including bone, brain, and reproductive organs, and plays a critical role in regulating the Wnt signaling pathway. DKK-1 functions by binding to the Wnt co-receptor LRP5/6, thereby inhibiting the canonical Wnt signaling cascade. By antagonizing Wnt signaling, DKK-1 suppresses the differentiation and proliferation of osteoblasts, leading to the inhibition of bone formation. DKK-1 is also involved in other biological processes, such as embryonic development, tissue homeostasis, and cancer progression. Dysregulation of DKK-1 has been implicated in skeletal disorders, such as osteoporosis and osteoarthritis, as well as in various cancers, highlighting the importance of DKK-1 in development and disease.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
