WB result of Dicer Recombinant Rabbit mAb
Primary antibody: Dicer Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Negative control: U937 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 219 kDa
Observed MW: 240 kDa
This blot was developed with high sensitivity substrate
Dicer Recombinant Rabbit mAb (S-2497-202)
Dicer Recombinant Rabbit mAb (S-2497-202)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Dicer |
| Synonyms | Endoribonuclease Dicer; Helicase with RNase motif (Helicase MOI); DICER; HERNA; KIAA0928; DICER1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm |
| Accession | Q9UPY3 |
| Clone Number | S-2497-202 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Ck, Hm, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Dicer is a multidomain RNase III enzyme that recognizes double-stranded RNA (dsRNA) or short-hairpin RNA (shRNA), measures ~22 nt from the 3′ overhang, and cleaves both strands to generate small interfering RNAs or microRNAs bearing 2-nt 3′ overhangs; these products are then loaded into Argonaute to form the RNA-induced silencing complex that drives gene silencing, making Dicer the central processor of the RNA interference pathway essential for development, viral defense, and genome stability.
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Western Blot
WB result of Dicer Recombinant Rabbit mAb
Primary antibody: Dicer Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 219 kDa
Observed MW: 240 kDa
This blot was developed with high sensitivity substrate
WB result of Dicer Recombinant Rabbit mAb
Primary antibody: Dicer Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat testis lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 220 kDa
Observed MW: 240 kDa
This blot was developed with high sensitivity substrate
