WB result of DHODH Recombinant Rabbit mAb
Primary antibody: DHODH Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 50 kDa
DHODH Recombinant Rabbit mAb (S-2055-30)
DHODH Recombinant Rabbit mAb (S-2055-30)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DHODH |
| Synonyms | Dihydroorotate dehydrogenase (quinone), mitochondrial; DHOdehase; Dihydroorotate oxidase |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q02127 |
| Clone Number | S-2055-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Rt |
| Positive Sample | MCF7, HepG2, C6, rat heart |
| Predicted Reactivity | Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Rt |
| ICC | 1:500 | Hu |
| ICFCM | 1:50 | Hu |
Background
Dihydroorotate dehydrogenase (DHODH) is a crucial enzyme located in the inner mitochondrial membrane, playing a vital role in the de novo pyrimidine biosynthesis pathway. It catalyzes the conversion of dihydroorotate to orotate, which is the fourth and only redox step in this pathway. This process is essential for the production of pyrimidine nucleotides, which are fundamental for DNA and RNA synthesis. DHODH is a flavoprotein that relies on ubiquinone (CoQ) for its function, and its activity is closely linked to the mitochondrial electron transport chain. Additionally, DHODH has garnered attention in cancer research due to its role in tumor cell proliferation and its potential as a target for cancer treatment.
Picture
Picture
Western Blot
WB result of DHODH Recombinant Rabbit mAb
Primary antibody: DHODH Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling DHODH antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in MCF7 cells. Anti- DHODH antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
