DGCR8 Recombinant Rabbit mAb (S-4082-6)

Catalog Number: S0B6932 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	DGCR8
Synonyms	Microprocessor complex subunit DGCR8; C22orf12; DGCRK6
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	Q8WYQ5
Clone Number	S-4082-6
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, Jurkat, HCT-116, HEK-293, A549, PC-3, Neuro-2a, NIH/3T3, mouse brain, PC-12, rat brain, rat testis
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

DGCR8 (DiGeorge Syndrome Critical Region 8), also known as Pasha in invertebrates, is an essential double-stranded RNA-binding protein that functions as a core component of the Microprocessor complex alongside the RNase III enzyme Drosha, playing a pivotal role in the canonical biogenesis of microRNAs (miRNAs) within the nucleus. By recognizing and binding to the apical loop and single-stranded RNA segments of primary miRNA transcripts (pri-miRNAs), DGCR8 serves as a molecular ruler that positions Drosha to precisely cleave the pri-miRNA base, thereby generating precursor miRNAs (pre-miRNAs) that are subsequently exported to the cytoplasm for further processing into mature miRNAs involved in post-transcriptional gene regulation. Beyond its central function in miRNA processing, DGCR8 contributes to the maintenance of genomic stability, DNA damage response, and cellular differentiation, and its haploinsufficiency due to deletions in chromosome 22q11.2 is directly linked to DiGeorge syndrome (22q11.2 deletion syndrome), a multisystem developmental disorder characterized by congenital heart defects, immune deficiencies, and neuropsychiatric abnormalities, underscoring the critical importance of precise DGCR8-mediated regulatory networks in human development and disease.

Picture

Western Blot

WB result of DGCR8 Recombinant Rabbit mAb
Primary antibody: DGCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Lane 6: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDa

WB result of DGCR8 Recombinant Rabbit mAb
Primary antibody: DGCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDa

WB result of DGCR8 Recombinant Rabbit mAb
Primary antibody: DGCR8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-DGCR8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.