采用Dead Cell Removal Set、S或L Separation Column及磁性分选器从C57BL/6小鼠脾细胞中去除死细胞。死细胞经碘化丙啶荧光染色后，使用FlowJo™软件进行分析。
Dead Cell Removal Set, (RUO)
Dead Cell Removal Set, (RUO)
Price:
Regular price
$335 USD
Regular price
Sale price
$335 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Stability & Storage | Store at 2–8°C protected from light. Do not freeze. |
Background
Dead Cell Removal Set provides a rapid and straightforward method to deplete dead cells from cell cultures or tissue preparations. The kit contains ready-to-use magnetic nanobeads and binding buffer for magnetic labeling of cell debris, dead cells and apoptotic cells. After magnetically removing labeled components, pure viable cells can be obtained within a short period of time.
Protocol
Protocol based on S Separation Column
Step | Procedure | Volume / Time | |
Magnetic Labeling | 1 | Count total cells Note: AO/PI staining is recommended for cell counting | — |
2 | Centrifuge cell suspension at 400×g for 7 min, aspirate supernatant completely | 400×g, 7 min | |
3 | Resuspend 1×10⁷ cells in 90 μL 1× Binding Buffer Note: Dilute 20× Binding Buffer to 1× Binding Buffer using sterile double-distilled water only, then supplement with 1% BSA before use | Cell resuspension | |
4 | Add 10 μL Dead Cell Removal NanoBeads per 1×10⁷ cells Note: Binding of Dead Cell Removal NanoBeads requires Ca²⁺. The presence of the ion chelator EDTA will interfere with binding | 10 μL per 1×10⁷ cells | |
5 | Mix thoroughly and incubate at 20–25°C for 15 min | Incubate for 15 min | |
6 | (Optional) If necessary, add 1× Binding Buffer to the cell suspension to reach a minimum volume of 500 μL for separation | — | |
Magnetic Separation | 7 | Place the S-type separation column into the magnetic field of the corresponding magnetic separator; rinse the column with 500 μL buffer | Column rinsing |
8 | Load cell suspension onto the column and collect flow-through (viable cells) | Collect viable cells | |
9 | Wash the column 3 times with buffer, 500 μL each time. Add the next aliquot only after the column reservoir is fully drained. Combine all flow-through as the negative fraction | Collect viable cells | |
10 | Remove the separation column from the magnetic field and place it above a collection tube | — | |
| 11 | Add 1 mL buffer to the column and rapidly push firmly with the supplied plunger to elute magnetically labeled positive cells Note: For higher purity, repeat separation once using a new column | Collect labeled cells |
Protocol based on L Separation Column
Step | Procedure | Volume / Time | |
Magnetic Labeling | 1 | Count total cells Note: AO/PI staining is recommended for cell counting | — |
2 | Centrifuge cell suspension at 400×g for 7 min, aspirate supernatant completely | 400×g, 7 min | |
3 | Resuspend 1×10⁷ cells in 90 μL 1× Binding Buffer Note: Dilute 20× Binding Buffer to 1× Binding Buffer using sterile double-distilled water only, then supplement with 1% BSA before use | Cell resuspension | |
4 | Add 10 μL Dead Cell Removal NanoBeads per 1×10⁷ cells Note: Binding of Dead Cell Removal NanoBeads requires Ca²⁺. The presence of the ion chelator EDTA will interfere with binding | 10 μL per 1×10⁷ cells | |
5 | Mix thoroughly and incubate at 20–25°C for 15 min | Incubate for 15 min | |
6 | (Optional) If necessary, add 1× Binding Buffer to the cell suspension to reach a minimum volume of 500 μL for separation | — | |
Magnetic Separation | 7 | Place the L-type separation column into the magnetic field of the corresponding magnetic separator; rinse the column with 3 mL buffer | Column rinsing |
8 | Load cell suspension onto the column and collect flow-through (viable cells) | Collect viable cells | |
9 | Wash the column 3 times with buffer, 3 mL each time. Add the next aliquot only after the column reservoir is fully drained. Combine all flow-through as the negative fraction | Collect viable cells | |
10 | Remove the separation column from the magnetic field and place it above a collection tube | — | |
| 11 | Add 5 mL buffer to the column and rapidly push firmly with the supplied plunger to elute magnetically labeled positive cells Note: For higher purity, repeat separation once using a new column | Collect labeled cells |
Picture
Picture
Validation Data
