DDX39B Recombinant Rabbit mAb (S-4549-30)

Catalog Number: S0B60321 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 1ml

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Product Details

Product Specification

Host	Rabbit
Antigen	DDX39B
Synonyms	Spliceosome RNA helicase DDX39B; 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein; BAT1; UAP56
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	Q13838
Clone Number	S-4549-30
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, K-562, Jurkat, HepG2, 293T, A431, NIH/3T3, RAW264.7, mouse colon, PC-12, C6
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

DDX39B (DEAD-box helicase 39B), also known as UAP56 or Bat1, is an ATP-dependent RNA helicase belonging to the DEAD-box family that participates in multiple critical steps of RNA metabolism within the nucleus. As an important component of the spliceosome B complex, DDX39B is responsible for regulating the recognition and pairing of U2 snRNP with the branch site during pre-mRNA splicing, ensuring accurate intron excision and exon ligation. Additionally, it plays a central role in mRNA nuclear export by interacting with the TREX (transcription-export) complex to transport mature mRNA from the nucleus to the cytoplasm for translation. In recent years, the functional scope of DDX39B has expanded to other biological areas, including participation in homologous recombination repair during DNA damage repair, as well as influencing cell proliferation and apoptosis through regulating the stability of specific mRNAs. Furthermore, DDX39B has been found to be associated with various diseases: it is overexpressed in multiple tumors (such as hepatocellular carcinoma and lung cancer), promoting tumor progression by enhancing the splicing and nuclear export of oncogenic mRNAs, and is correlated with poor patient prognosis. In viral infections, certain viruses hijack DDX39B to facilitate the processing and nuclear export of their own RNAs. Meanwhile, polymorphisms in the DDX39B gene have also been implicated in susceptibility to autoimmune diseases such as rheumatoid arthritis, highlighting the bridging role of this protein between fundamental RNA metabolism and disease development.

Picture

Western Blot

WB result of DDX39B Recombinant Rabbit mAb
Primary antibody: DDX39B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K-562 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lane 6: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDa

WB result of DDX39B Recombinant Rabbit mAb
Primary antibody: DDX39B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW.264.7 whole cell lysate 20 µg
Lane 3: mouse colon lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDa

WB result of DDX39B Recombinant Rabbit mAb
Primary antibody: DDX39B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brain. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-DDX39B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.