The CRBN/DDB1 activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration of lenalidomide, CRBN/DDB1 protein, substrate and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
DDB1/CRBN Complex, His Tag Protein
DDB1/CRBN Complex, His Tag Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | DDB1/CRBN Complex |
| Accession | Q16531-1、Q96SW2-1 |
| Amino Acid Sequence |
CRBN(M1-L442 end) N-terminal 6x His tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 53.7 KDa/126.9 KDa |
| Purity | >85% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 3 μg CRBN/DDB1
