Dctd Recombinant Rabbit mAb (S-4313-16)

Catalog Number: S0B60104 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	Dctd
Synonyms	Deoxycytidylate deaminase; DCTD
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	P32321
Clone Number	S-4313-16
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	MCF7, BxPC-3, SK-OV-3, U-87 MG, HT-1080
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu
IHC-P	1:200	Hu

Background

DCTD, also known as dCMP deaminase, is encoded by the human DCTD gene and is a homotypic hexameric enzyme that plays a critical role in pyrimidine nucleotide metabolism. This protein is approximately 178 amino acids in length with a molecular weight of about 20 kDa, is primarily localized in the cytoplasm, and its enzymatic activity depends on zinc ions as a cofactor. Functionally, DCTD catalyzes the hydrolytic deamination of deoxycytidylate (dCMP) to convert it into deoxyuridylate (dUMP), which is the direct substrate for thymidylate synthase in the synthesis of dTMP. Therefore, DCTD provides the essential precursors for DNA replication and repair through this key step. The activity of this enzyme is tightly regulated by allosteric mechanisms, with its activator dCTP enhancing its activity while the end product dTTP inhibits it, thereby precisely maintaining the balance of the intracellular deoxynucleotide pool. In addition, the DCTD protein undergoes various post-translational modifications, including phosphorylation, ubiquitination, and acetylation, which may participate in the fine-tuning of its activity and function.

Picture

Western Blot

WB result of Dctd Recombinant Rabbit mAb
Primary antibody: Dctd Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 24 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-Dctd antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-Dctd antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-Dctd antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.