1 1

DCAF15/DDB1 His&StrepII Tag Protein, Human

DCAF15/DDB1 His&StrepII Tag Protein, Human

Catalog Number: UA080523 Brand: UA BIOSCIENCE
Share:
Price:
$500 USD
$500 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Accession Q66K64、Q16531
Amino Acid Sequence

A2-L600 end (DCAF15), N-His-StrepII-TEV-GG
M1-H1140 end (DDB1), No tag
Expression System Baculovirus-InsectCells
Molecular Weight 69.6 KDa/126.9 KDa
Conjugation Unconjugated
Tag His Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT
Stability & Storage

-80℃

Guidelines

Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!

Picture

Bioactivity

Gel based ligase assay
Lane M: Marker
Lane 1: Bodipy-Ub + UBE1 + Ubch5b + DCAF15/DDB1 with ATP
Lane 2: Bodipy-Ub + UBE1+ Ubch5b with ATP
Lane 3: Bodipy-Ub + UBE1+ Ubch5b without ATP
The ubiquitin conjugating activity of DCAF15/DDB1 was validated through its ability to catalyse
the generation of polyubiquitin chains in the presence of the E1 activating enzyme UBE1, the E2
conjugating enzyme Ubch5b and Bodipy-ubiquitin. Incubation of DCAF15/DDB1 for 60 minutes at
37˚C in the presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP (Lane 1) was compared
alongside two control reactions with either DCAF15/DDB1 (Lane 2) or DCAF15/DDB1&ATP
(Lane 3) excluded from the reaction. Ubiquitin conjugates were identified by the migration of the
Bodipy-ubiquitin band and these were observed only in the presence of ATP and DCAF15/DDB1.

SDS-PAGE

Lane M: Marker
Lane 1: 2 μg, reduced
Lane 2: 2 μg, non-reduced