mIHC shows positive staining in paraffin-embedded human tonsil. Anti-CD8α antibody (S0B2148) was used at 1/500 dilution (Green), Anti-Granzyme B antibody (S0B0359) was used at 1/200 dilution (Red), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Product Details
Product Details
Product Specification
Antibody Type | Recombinant mAb |
Application | mIHC |
Reactivity | Hu |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
application | dilution | species |
mIHC | 1:200-1:500 |
Background
Cytotoxic T cell and natural killer (NK) cell (CD8α, Granzyme B) Antibody Panel contains 2 recombinant monoclonal antibodies against CD8α, Granzyme B. They are provided as a sampler panel.
Panel contains:
- Granzyme B Recombinant Rabbit mAb (S-357-54) S0B0359
- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148
Picture
Picture
Multiple Immunohistochemistry
