CYP1B1 Recombinant Rabbit mAb (S-6022)

CYP1B1, full name Cytochrome P450 Family 1 Subfamily B Member 1, is a heme-thiolate monooxygenase belonging to the cytochrome P450 superfamily, encoded by the CYP1B1 gene. It is primarily localized on the endoplasmic reticulum membrane and is expressed in tissues such as the eye, heart, brain, lung, and kidney, as well as in various tumor cells. As a phase I metabolic enzyme, the core function of CYP1B1 is to catalyze the oxidative metabolism of various exogenous chemicals (such as polycyclic aromatic hydrocarbons, aromatic amines, and heterocyclic amines) and endogenous substrates (such as 17β-estradiol and arachidonic acid) in a NADPH- and molecular oxygen-dependent manner. While participating in the detoxification of foreign substances, it can also activate procarcinogens into genotoxic intermediates, thereby contributing to chemical carcinogenesis. At the physiological level, CYP1B1 plays a critical role in eye development; it participates in the morphogenesis of the retina and cornea and regulates intraocular pressure through the metabolism of endogenous molecules such as retinoic acid and arachidonic acid. Loss-of-function mutations in CYP1B1 are among the most important causative factors for primary congenital glaucoma (PCG), leading to impaired aqueous humor outflow and elevated intraocular pressure via affecting the development of the trabecular meshwork and Schlemm's canal, ultimately resulting in optic nerve damage. Furthermore, CYP1B1 expression is transcriptionally regulated by the aryl hydrocarbon receptor (AhR) and is aberrantly overexpressed in many malignancies (such as breast cancer, prostate cancer, lung cancer, and colorectal cancer). Through metabolic activation of procarcinogens and promotion of estrogen-mediated genotoxicity, it is closely associated with tumor initiation, progression, and drug resistance, making it a potential target of great interest in cancer prevention and therapy.