Cyclin E1 Recombinant Rabbit mAb (SDT-4375-44)

Catalog Number: S0B2493 Application: WB, IHC-P, ICC Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 500μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Cyclin E1
Synonyms	G1/S-specific cyclin-E1; CCNE; CCNE1
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P24864
Clone Number	SDT-4375-44
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu
Positive Sample	MCF7, HepG2, HT-29, PC-3, HCT 116, OVCAR3, Jurkat, HeLa, JAR, K-562
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500	Hu
ICC	1:100	Hu

Background

Cyclin E1 is a regulatory subunit of the cyclin-dependent kinase Cdk2 that drives the G1-S phase transition by phosphorylating substrates such as the retinoblastoma protein, thereby releasing E2F transcription factors and committing cells to DNA replication; its expression peaks in late G1, is tightly controlled by ubiquitin-mediated proteolysis, and is frequently amplified or overexpressed in cancers, leading to genomic instability and aggressive tumor behavior.

Picture

Western Blot

WB result of Cyclin E1 Recombinant Rabbit mAb
Primary antibody: Cyclin E1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Lane 6: OVCAR3 whole cell lysate 20 µg
Lane 7: Jurkat whole cell lysate 20 µg
Lane 8: HeLa whole cell lysate 20 µg
Lane 9: JAR whole cell lysate 20 µg
Lane 10: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 38-47 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human placenta. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in K-562 cells. Anti-Cyclin E1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG-H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).