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Cyclin E1 Recombinant Rabbit mAb (SDT-4375-44)

Cyclin E1 Recombinant Rabbit mAb (SDT-4375-44)

Catalog Number: S0B2493 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Cyclin E1
Synonyms G1/S-specific cyclin-E1; CCNE; CCNE1
Immunogen Synthetic Peptide
Location Nucleus
Accession P24864
Clone Number SDT-4375-44
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample MCF7, HepG2, HT-29, PC-3, HCT 116, OVCAR3, Jurkat, HeLa, JAR, K-562
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:500 Hu

Background

Cyclin E1 is a regulatory subunit of the cyclin-dependent kinase Cdk2 that drives the G1-S phase transition by phosphorylating substrates such as the retinoblastoma protein, thereby releasing E2F transcription factors and committing cells to DNA replication; its expression peaks in late G1, is tightly controlled by ubiquitin-mediated proteolysis, and is frequently amplified or overexpressed in cancers, leading to genomic instability and aggressive tumor behavior.

Picture

Western Blot


WB result of Cyclin E1 Recombinant Rabbit mAb
Primary antibody: Cyclin E1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Lane 6: OVCAR3 whole cell lysate 20 µg
Lane 7: Jurkat whole cell lysate 20 µg
Lane 8: HeLa whole cell lysate 20 µg
Lane 9: JAR whole cell lysate 20 µg
Lane 10: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 38-47 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human placenta. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.