Cullin4A-RBX1-DDB1-CRBN Protein

Catalog Number: UA080275 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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Product Details

Product Specification

Species	Human
Synonyms	CUL4A/RBX1/DDB1/CRBN Complex
Amino Acid Sequence	His-RBX1 (full-length), HA-DDB1 (full-length), Cullin4A (full-length), CRBN (full-length)
Expression System	Baculovirus-InsectCells
Purity	＞90% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	50 mM Tris, 200 mM NaCl, 2 mM TCEP, pH 8.0
Stability & Storage	Store at -80ºC. Avoid multiple freeze-thaw cycles.

Background

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes. In the DCX complex (DDB1-CUL4-X-box), CUL4A serves as a scaffold that organizes the DDB1-X-box substrate recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the CUL4 subunit, though neddylation may be dispensable in some in vitro reactions.

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Bioactivity

The Cullin4A-RBX1-DDB1-CRBN activity was measured by protein-tracer binary binding assay using HTRFtechnology.

The Cullin4A-RBX1-DDB1-CRBN activity was measured by PROTAC-mediated Ternary Complex Formation using HTRFtechnology. The dose response curve of dBET1 in the Ternary Assay exhibited a bell-shaped curve, a hook effect and a peak.

The Cullin4A-RBX1-DDB1-CRBN activity was measured by WB analysis for target protein ubiquitination assay. The Ubiquitinated BRD4(D1+D2) bands increased with time.