CTBP1 Recombinant Rabbit mAb (S-4463-63)

Catalog Number: S0B60763 Application: WB, ICC Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	CTBP1
Synonyms	C-terminal-binding protein 1; CTBP
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	Q13363
Clone Number	S-4463-63
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, ICC
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	SH-SY5Y, NIH/3T3, Neuro-2a, mouse brain, mouse thymus, rat brain, rat thymus, COS-7
Predicted Reactivity	Xe
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
ICC	1:100	Hu, Ms

Background

CTBP1 (C-terminal binding protein 1) is a transcriptional corepressor that is highly conserved across metazoans, named for its initial discovery as a protein that binds to the C-terminal region of the adenoviral E1A oncoprotein. Structurally, CTBP1 shares extensive homology with the D-2-hydroxyacid dehydrogenase family and contains a conserved NAD(H)-binding domain and a Rossmann fold; however, dehydrogenase activity in vitro is not required for its transcriptional repression function—rather, the conformational changes induced by NAD(H) binding and oligomerization (especially tetramer formation) are key to its corepressor activity. CTBP1 primarily recognizes and binds the PXDLS motif present in many cellular transcription factors through a hydrophobic cleft at its N-terminus, and then acts as a scaffold to recruit chromatin-modifying enzymes such as histone deacetylases, methyltransferases, and demethylases to the promoters of target genes, repressing the expression of numerous targets, including genes involved in apoptosis, cell adhesion, and epithelial–mesenchymal transition. In addition, CTBP1 participates in extranuclear processes such as Golgi membrane fission and brown adipocyte differentiation, and its dysregulated expression is associated with poor prognosis in multiple cancers, making it a potential drug target.

Picture

Western Blot

WB result of CTBP1 Recombinant Rabbit mAb
Primary antibody: CTBP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 45 kDa

WB result of CTBP1 Recombinant Rabbit mAb
Primary antibody: CTBP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 45 kDa

WB result of CTBP1 Recombinant Rabbit mAb
Primary antibody: CTBP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 45 kDa

WB result of CTBP1 Recombinant Rabbit mAb
Primary antibody: CTBP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 45 kDa

Immunocytochemistry

ICC shows positive staining in SH-SY5Y cells. Anti-CTBP1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ICC shows positive staining in NIH/3T3 cells. Anti-CTBP1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).