S-RMabMixTM Crotonyllysine Rabbit mAb

Catalog Number: S0B1324 Application: WB Brand: Starter

Price:

$160 USD

Size: 25μl 100μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	Crotonyllysine
Synonyms	Kcr, 巴豆酰化
Immunogen	Synthetic Peptide
Antibody Type	Recombinant mAb Mix
Isotype	IgG
Application	WB
Purification	Protein A
Concentration	5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS pH7.4
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution

application	dilution	species
Dot Blot	1:5000
WB	1:1000	Hu, Ms

Background

Crotonyllysine is a post-translational modification of protein lysine residues, characterized by the addition of a crotonyl group. This modification plays a crucial role in various cellular processes, including gene transcription, acute kidney injury, and spermatogenesis. The dynamic balance of crotonyllysine modification is regulated by a set of enzymes, including "writers" that add the modification and "erasers" that remove it. Crotonyl-CoA, the primary precursor for crotonyllysine modification, is influenced by the activity of enzymes such as ACSS2 and CDYL. Abnormal levels of crotonyl-CoA and crotonyllysine have been implicated in several diseases, including cancer and cardiovascular diseases, making it an important area of research for future therapeutic strategies.

Picture

Western Blot

WB result of S-RMabMix™ Crotonyllysine Rabbit mAb
Primary antibody: S-RMabMix™ Crotonyllysine Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

WB result of S-RMabMix™ Crotonyllysine Rabbit mAb
Primary antibody: S-RMabMix™ Crotonyllysine Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whloe cell lysate 20 µg
Lane 2: HeLa treated with 50 mM sodium crotonate for 24 hours whloe cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

Dot Blot

Dot blot result of S-RMabMix™ Crotonyllysine Rabbit mAb
Lane 1: Modified peptide library (Crotonyl lysine)
Lane 2: Unmodified peptide library control
Lane 3: Modified peptide library (Methacryl lysine)
Lane 4: Modified peptide library (Fumaryl lysine)
Lane 5: Modified peptide library (Succinyl lysine)
Lane 6: Modified peptide library (Butyryl lysine)
Lane 7: Modified peptide library (L-lactyl lysine)
Primary antibody: S-RMabMix™ Crotonyllysine Rabbit mAb at 1/5000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution