WB result of CREB (phospho S133) Recombinant Rabbit mAb
Primary antibody: CREB (phospho S133) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A for 15 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 45 kDa
CREB (phospho S133) Recombinant Rabbit mAb (S-1100-102)
CREB (phospho S133) Recombinant Rabbit mAb (S-1100-102)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CREB (phospho S133) |
| Synonyms | Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P16220-1 |
| Clone Number | S-1100-102 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IHC-P | 1:200 |
Background
CREB (cAMP response element-binding protein) is a well-known transcription factor that plays a pivotal role in the regulation of gene expression in response to various stimuli, including neuronal activity, hormones, and growth factors. Phosphorylation of CREB at serine 133 (Ser133) is a critical event for its activation, as it enhances the binding of CREB to the cAMP response element (CRE), leading to the transcription of target genes involved in processes such as learning, memory, and cell survival. The phosphorylation of CREB at Ser133 is typically catalyzed by various protein kinases, including protein kinase A (PKA), calcium/calmodulin-dependent protein kinase IV (CaMKIV), and mitogen-activated protein kinase (MAPK). This phosphorylation event is a robust indicator of neuronal activation and synaptic plasticity, making it a widely studied marker in neurobiological research.
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Western Blot
Dot Blot
Dot blot result of CREB (phospho S133) Recombinant Rabbit mAb
Lane 1: CREB (S133) phospho peptide
Lane 2: CREB unmodified peptide
Primary antibody: CREB (phospho S133) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
