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CRBN/DDB1 Protein, Mouse

CRBN/DDB1 Protein, Mouse

Catalog Number: UA080515 Brand: UA BIOSCIENCE
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$580 USD
$580 USD
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Product Details

Product Specification


Species Mouse
Accession Q8C7D2-1、Q3U1J4
Amino Acid Sequence

CRBN:M1-L445(end); N-terminal 8x His tag
DDB1: M1-H1140(end);no tag
Expression System Baculovirus-InsectCells
Molecular Weight

CRBN: 53.3 kDa; DDB1: 126.9 kDa
Purity > 70% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag, No Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

Picture

Bioactivity

The CRBN/DDB1 activity was detected using TR-FRET technology. The reaction was performed by incubating the CRBN/DDB1 protein and Thalidomide-BODIPY, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 520/490 with BMG.

SDS-PAGE