CRBN GST&His Tag Protein, Human

Catalog Number: UA070005 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$313.00 SGD

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Product Details

Product Specification

Species	Human
Synonyms	CRBN, AD-006, MRT2, MRT2A
Accession	Q96SW2
Amino Acid Sequence	Met1-Leu442, with N-terminal GST MSGSHHHHHHSSGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLGHTGHRSGDDDDKMAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
Expression System	E.coli
Molecular Weight	76-78kDa (Reducing)
Purity	>90% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Tag	His Tag, GST Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Stability & Storage	· 12 months from date of receipt, -20 to -70 °C as supplied. · 6 months, -20 to -70 °C under sterile conditions after reconstitution. · 1 week, 2 to 8 °C under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	lioua, A. , Kundu, P. , Lu, R. , Kumar, Y. , & Toro, L. . (2009). Large conductance calcium-activated potassium channels. Encyclopedia of Neuroence, 373-381. Higgins, J. J. , Pucilowska, J. , Lombardi, R. Q. , & Rooney, J. P. . (2004). A mutation in a novel atp-dependent lon protease gene in a kindred with mild mental retardation. Neurology, 63(10), 1927-1931.

Background

Cereblon (CRBN; a member of the ATP-dependent Lon protease) is an enzyme implicated in memory and learning. Interestingly, CRBN and K_Ca1.1 are both highly expressed in the same areas of the brain, including the hippocampus, which is known for its involvement in memory and learning. These two proteins bind directly to each other via the C-terminus of K_Ca1.1 in vitro, and their proximity in brain has been confirmed biochemically and by visualization of immunolabeled complexes in cultured hippocampal neurons. Ectopically expressed CRBN suppresses K_Ca1.1 surface expression by disrupting the formation of tetramers and therefore functional channels. The physiological consequences of the interaction between CRBN and KCa1.1 remain to be explored, although inherited mutations of the human cereblon gene (CRBN) or K_Ca1.1 lead to mental retardation disorders. And substrate recognition component of a DCX (DDB1-CUL4-X- Box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Regular degradation of Key regulatory proteins is necessary for Normal limb outgrowth and expression of the fibroblast growth factor FGF8.

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SDS-PAGE

2μg (R: reducing conditions, N: non-reducing conditions).

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CRBN GST&His Tag Protein, Human