COXIV Recombinant Rabbit mAb (S-5358)

Catalog Number: S0B60175 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	COXIV
Synonyms	Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX4; COX4I1
Location	Mitochondrion
Accession	P13073
Clone Number	S-5358
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, HepG2, A431, Jurkat, MCF7, mouse liver, rat liver, rat skeletal muscle, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:10000	Hu, Ms, Rt, Mk
IHC-P	1:2000	Hu, Ms, Rt

Background

COXIV is a key subunit of cytochrome c oxidase (Complex IV), the final enzyme complex in the mitochondrial respiratory chain. This protein consists of approximately 160 amino acids, containing an N-terminal mitochondrial targeting sequence and multiple α-helical transmembrane domains, and is primarily anchored in the inner mitochondrial membrane. COXIV serves dual important functions—structural and regulatory: on one hand, it provides structural support for the core catalytic subunits (COX I and COX II) of Complex IV, assisting in the assembly and stability of the entire enzyme complex; on the other hand, it senses the cellular energy state through an ATP/ADP binding site, thereby allosterically regulating the catalytic activity of Complex IV to match the efficiency of electron transfer in the respiratory chain with ATP synthesis demands. Furthermore, COXIV expression levels vary across different tissues (such as the liver-type COXIV-1 and lung-type COXIV-2 isoforms), and its expression is regulated by transcription factors such as hypoxia-inducible factor HIF-1α, playing a role in cellular responses to hypoxic stress and metabolic reprogramming. At the pathological level, mutations or aberrant expression of COXIV are closely associated with various mitochondrial diseases, including mitochondrial encephalomyopathies and Leigh syndrome. Its dysfunction leads to decreased electron transport chain efficiency and excessive production of reactive oxygen species (ROS), making COXIV also an important potential target in research on ischemia-reperfusion injury, neurodegenerative diseases, and tumor metabolism.

Picture

Western Blot

WB result of COXIV Recombinant Rabbit mAb
Primary antibody: COXIV Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 16 kDa

WB result of COXIV Recombinant Rabbit mAb
Primary antibody: COXIV Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 16 kDa

WB result of COXIV Recombinant Rabbit mAb
Primary antibody: COXIV Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 16 kDa

WB result of COXIV Recombinant Rabbit mAb
Primary antibody: COXIV Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 16 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.