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COXIV Recombinant Mouse mAb (S-5528)

COXIV Recombinant Mouse mAb (S-5528)

Catalog Number: S0B60250 Application: WB, IHC-P Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen COXIV
Synonyms Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX4; COX4I1
Location Mitochondrion
Accession P13073
Clone Number S-5528
Antibody Type Mouse mAb
Isotype IgG1
Application WB, IHC-P
Reactivity Hu, Mk
Positive Sample HeLa, HepG2, A431, Jurkat, MCF7, LnCap, COS-7
Purification Protein G
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:5000-1:50000 Hu, Mk
IHC-P 1:2000 Hu

Background

COXIV is a key subunit of cytochrome c oxidase (Complex IV), the final enzyme complex in the mitochondrial respiratory chain. This protein consists of approximately 160 amino acids, containing an N-terminal mitochondrial targeting sequence and multiple α-helical transmembrane domains, and is primarily anchored in the inner mitochondrial membrane. COXIV serves dual important functions—structural and regulatory: on one hand, it provides structural support for the core catalytic subunits (COX I and COX II) of Complex IV, assisting in the assembly and stability of the entire enzyme complex; on the other hand, it senses the cellular energy state through an ATP/ADP binding site, thereby allosterically regulating the catalytic activity of Complex IV to match the efficiency of electron transfer in the respiratory chain with ATP synthesis demands. Furthermore, COXIV expression levels vary across different tissues (such as the liver-type COXIV-1 and lung-type COXIV-2 isoforms), and its expression is regulated by transcription factors such as hypoxia-inducible factor HIF-1α, playing a role in cellular responses to hypoxic stress and metabolic reprogramming. At the pathological level, mutations or aberrant expression of COXIV are closely associated with various mitochondrial diseases, including mitochondrial encephalomyopathies and Leigh syndrome. Its dysfunction leads to decreased electron transport chain efficiency and excessive production of reactive oxygen species (ROS), making COXIV also an important potential target in research on ischemia-reperfusion injury, neurodegenerative diseases, and tumor metabolism.

Picture

Western Blot


WB result of COXIV Recombinant Mouse mAb
Primary antibody: COXIV Recombinant Mouse mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: LnCap whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 17 kDa

WB result of COXIV Recombinant Mouse mAb
Primary antibody: COXIV Recombinant Mouse mAb at 1/5000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 17 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-COXIV antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.