COX2 / Cyclooxygenase Recombinant Rabbit mAb (S-5952)

Catalog Number: S0B60390 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	COX2
Synonyms	Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II; COII; COXII; MTCO2; MT-CO2
Location	Mitochondrion
Accession	P00405
Clone Number	S-5952
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	U-87 MG, Raw 264.7 treated with LPS (1μg/mL, 24 hours)
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

MTCO2 (Mitochondrially Encoded Cytochrome C Oxidase II, also known as COX2 or COII) is a ~25 kDa protein encoded by the mitochondrial genome and embedded in the inner mitochondrial membrane as one of the three mitochondrially-encoded core subunits of cytochrome c oxidase (Complex IV), the terminal enzyme complex of the electron transport chain. This protein plays a critical role in aerobic respiration by mediating the transfer of electrons from cytochrome c to molecular oxygen via its binuclear copper A center (CuA), ultimately catalyzing the reduction of oxygen to water while contributing to the generation of the proton gradient essential for ATP synthesis through oxidative phosphorylation. Highly expressed in energy-demanding tissues such as cardiac muscle, skeletal muscle, and neurons, MTCO2 functions as a pivotal component of the respiratory chain, and its mutations or dysfunctions have been implicated in various mitochondrial disorders, including mitochondrial myopathy, Leber's hereditary optic neuropathy, and other conditions arising from compromised oxidative phosphorylation and cellular energy failure.

Picture

Western Blot

WB result of COX2 / Cyclooxygenase Recombinant Rabbit mAb
Primary antibody: COX2 / Cyclooxygenase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 70 kDa

WB result of COX2 / Cyclooxygenase Recombinant Rabbit mAb
Primary antibody: COX2 / Cyclooxygenase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Raw 264.7 whole cell lysate 20 µg
Lane 2: Raw 264.7 treated with LPS (1μg/mL, 24 hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 65 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-COX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.