WB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: A-673 whole cell lysate 20 µg
Lane 3: MOLT-4 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: HT-29 whole cell lysate 20 µg
Lane 7: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
CoREST Recombinant Rabbit mAb (S-4401-8)
CoREST Recombinant Rabbit mAb (S-4401-8)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CoREST |
| Synonyms | REST corepressor 1; KIAA0071; RCOR; RCOR1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9UKL0 |
| Clone Number | S-4401-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HL-60, A-673, MOLT-4, Jurkat, HeLa, HT-29, HEK-293, A20, C2C12, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
The CoREST protein (primarily encoded by the RCOR1 gene, also known as CoREST1) was initially identified as a key corepressor of the transcriptional repressor REST (RE1-Silencing Transcription Factor), functioning by binding to REST to silence neuron-specific gene expression in non-neuronal cells, thereby playing an important role in maintaining cellular identity. Structurally, the CoREST protein contains two SANT domains and an ELM2 domain, which serve as the fundamental basis for its role as a molecular scaffold in recruiting histone-modifying enzymes. Its most distinctive function is to act as the core scaffold of the LSD1-CoREST-HDAC complex, simultaneously binding the histone demethylase LSD1 and the histone deacetylases HDAC1/2, thereby integrating two distinct epigenetic modifying enzymes into a single complex to coordinately regulate chromatin status and gene transcription. Notably, the human genome contains three CoREST family members (CoREST1, CoREST2, and CoREST3), which exhibit differences in their binding affinities for LSD1 and HDAC as well as their transcriptional repressive activities—for instance, CoREST2 binds more weakly to HDAC1/2 due to specific amino acid variations, endowing different complexes with the capacity for fine-tuned regulation of gene expression. Furthermore, recent studies have revealed that CoREST proteins function far beyond the scope of "REST corepressors," as they can independently regulate biological processes such as neural development in a REST-independent manner and play important roles in various diseases.
Picture
Picture
Western Blot
WB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
WB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
WB result of CoREST Recombinant Rabbit mAb
Primary antibody: CoREST Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CoREST antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
